![]() Furthermore, markedly decreased activities of glutathione peroxidise, superoxide dismutase, catalase and glutathione content was identified in ALC rats however GTE supplementation significantly (P<0.05) restored these levels close to normal. MtDNA deletions were observed in ALC rats, but intact native mtDNA was found in ALC+GTE group suggesting alcohol induced oxidative damage of mtDNA and ameliorative effect of GTE. Interestingly, D-loop mutations observed in ALC rats were successfully restored on GTE supplementation. DNA mfold analysis predicted higher free energy for 15507C and 16116C alleles compared to their corresponding wild alleles which represents less stable secondary structures with negative impact on overall mtDNA function. Results showed increased frequency of D-loop mutations in alcoholic rats (ALC). In addition, mtDNA deletions and antioxidants activity was measured in hepatic tissue of all rats. Entire displacement loop (D-loop) of mtDNA was screened by PCR-Sanger’s sequencing method. MtDNA was isolated from hepatic tissues of albino wistar rats after alcohol treatment with and without GTE supplementation. The present study principally sought to investigate the effect of green tea extract (GTE) supplementation on hepatic mitochondrial DNA (mtDNA) damage in alcohol receiving rats. ![]()
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February 2023
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